Plant Transcription Factor Database
Previous version: v3.0
Citrus clementina
HSF Family
Species TF ID Description
Ciclev10001482mHSF family protein
Ciclev10005403mHSF family protein
Ciclev10005649mHSF family protein
Ciclev10006893mHSF family protein
Ciclev10006902mHSF family protein
Ciclev10008116mHSF family protein
Ciclev10008617mHSF family protein
Ciclev10008618mHSF family protein
Ciclev10008768mHSF family protein
Ciclev10011531mHSF family protein
Ciclev10011698mHSF family protein
Ciclev10015413mHSF family protein
Ciclev10015472mHSF family protein
Ciclev10015540mHSF family protein
Ciclev10015541mHSF family protein
Ciclev10016707mHSF family protein
Ciclev10020718mHSF family protein
Ciclev10020724mHSF family protein
Ciclev10021287mHSF family protein
Ciclev10026058mHSF family protein
Ciclev10026273mHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645